In UV, the absorbance peak was observed at 531nm, FTIR spectrum shows the wavelength range from 464.27 cm-1 to 3364.51cm-1 and SEM image showed that gold nanoparticles are in spherical shape. The synthesized gold nanoparticles were characterized using UV-Visible spectroscopy, Fourier Transform Infrared spectroscopy and Scanning Electron Microscope. The green synthesis of gold nanoparticles using the aqueous root extract of Anacardium occidentale by bio-reduction of Auric chloride solution was investigated in the current study. Nanoparticles are being considered as elemental building blocks of nanotechnology. First Name Middle name Last name Father/husband first name Address State District PIN Code N NA No 363, 8th Main Road 7th Cross, ST Bed Koramangala Extn Bangalore 77 PARDUMAN NAGAR NEW AVAS VIKAS SAHARANPUR KARNATAKA UTTAR PRADESH BANGALORE SAHARANPUR 560034 IN30009510351656 247001 1201910100114010 720.00 1 150.00 1 NA AT POST PURAR, TAL MANGAON DIST.
9386223007, 9789386223005. Facebook gives people the.ISBN. Join Facebook to connect with Krishnasri Gadde and others you may know. KEYWORDS:View the profiles of people named Krishnasri Gadde.
It was thickly populated during proto-historic period and the present study is spatio-temporal analysis. The Chutang is now an extinct river represented by a dry channel flowing through parts of Haryana and Rajasthan. Science & Nature / Water Studies.
It contain riboflavin, E,C and K vitamins, antifungal, antiparasitic and anti-inflammatory properties. To investigate furthermore applications in gold nanoparticles, it is synthesized via green method from Anacardium occidentale whose common name is cashew plant. The gold nanoparticles with 1nm to 8nm are used in diagnosis of heart diseases and cancers as the biomarkers, as good biological imaging applications. The nanoparticle synthesis using phyto extracts have been creating waves of spirit of eco friendliness and its application in many fields specially concentrating in medical field 8-10. The nanoparticles are the one which are very tiny and are of the size 1 to 100 nm with numerous applications which are synthesized by three methods – which are Physical, Chemical and Biological method 5-7. So to deal with it, we are conjoining the nanoparticles with these substances so as to be eco-friendly, to increase the efficiency of insulin and also to increase the normally metabolism of human body 1-4.
Auric chloride solution was prepared by weighing for which the concentration ought to be 1Mm and is dissolved in double dissolved water in a 50ml standard flask. The aqueous extract was prepared from the dried sample by boiling it with water which is the universal solvent, it was then filtered by using whatmann no 40 filter paper and the stock solution was collected in a brown bottle and is then refrigerated.The stock solution was prepared from the dried roots of Anacardium occidentale using water as the solvent for the preparation of the aqueous extract. After the collection, the roots were shade dried well and made into the powder sample 15-16. They are washed well with normal water and then with double distilled water.
Krishnaveni Gaddewar Free Radical Scavenging
The working of the SEM is by the use narrow beam of highly energytic electron at the surface of the sample to give the signals which are in turn giving the information about the topographical, morphological and compositional informations.Antioxidant Activity (Dpph Free Radical Scavenging Activity) DeterminationHere the antioxidant activity of gold nanoparticles was studied using the DPPH stability by examining the scavenging effect on the free radical. The principle of the SEM is same as that of the optical microscope, but it measures the electrons scattered from the sample rather than that of the photons. By doing this FTIR measurement, the molecules that act as the reducing and stabilising agents are identified.The Scanning Electron Microscope is employed for the determination of the morphology of the synthesized metal nanoparticles. This concept is mainly based on the Beer-Lambert law which states the absorbance is directly proportional to the concentration of absorbing particles present in the solution.The formations of the nanoparticles synthesized were confirmed by the measurement of the SPR band using the U-2900 spectrophotometer with 1 cm path length in a 2ml quartz cuvette.FTIR is usually done to analyse the various bioconstituents present in the plant sample, so that it is easy to identify what type of compounds present in the extract. At about 30 seconds after the addition, the colour of the solution changes from pale yellow to ruby red colour indicating the formation of stable gold nanoparticles.The main purpose of using UV-Visible spectroscopy is to determine the optical properties of the solution.
The solution of p-nitrophenyl glucopyranoside (p-NPG) was added to pH 6.9. A graph was plotted using percent inhibition versus concentration curve and the inhibiton for 50% was determined.The α-glucosidase activity of the synthesized gold nanoparticle was studied. The mixture was shaken vigorously and left undisturbed for 5 min and using the spectrophotometer, the absorbance was measured at 540nm.
The Anacardium occidentale root extract shows the following list of phytoconstituents like resin, phenol, quinines, glycosides, flavanoids, sapanoids, proteins etc.Table 1: Qualitative Analysis of Anacardium occidentale Root extractThe gold nanoparticles were synthesised by taking the auric chloride solution of 1 mM concentration and thus by gradually adding the Anacardium occidentale Root extract toit. The 50% inhibition of enzyme activity was derived graphically.The quantitative analysis of Anacardium occidentale root extract was done and the results are tabulated in table 1. Here also the inhibition was studied by the inhibition formula. The yellow colour of the solution determines activity. It was then incubated for 20 min at temperature 37✬ and the reaction was brought to an end by adding sodium carbonate. The reaction is started by adding the nanoparticles dissolved in buffer.
Thus, by the periodic observation by using UV-Visible spectroscopy, the bio-reduction of the nanoparticles due to which the auric chloride changed its colour from colorless to ruby red.Figure 1: UV-Visible spectra of gold nanoparticles.FTIR is usually done to analyse the various bioconstituents present in the Anacardium occidentale Root extract and to identify what type of compounds present in the extract which are acts as the reducing and stabilizing agents.F igure 2: FTIR of Anacardium occidentale root extractF igure 3: FTIR spectrum of synthesized gold nanoparticles.Table 2: Comparision of FTIR of the Anacardium occidecntal root extract and gold nanoparticle. These are due to the colour change in the nanoparticles which was observed due to the shift resulted from the Surface Plasmon Resonance (SPR) band. This colour remained unchanged for more than a month which was periodically checked at regular intervals.The absorbance peak for gold nanoparticles was observed at 531nm.
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